A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans

作者：Huang C J; Butler A E; Moran A; Rao P N; Wagner J E; Blazar B R; Rizza R A; Manivel J C; Butler P C^*

来源：Diabetologia, 2011, 54(5): 1066-1074.

DOI：10.1007/s00125-011-2071-2

• Beta cell
• Cord blood cell
• Diabetes
• Stem cell

We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans.
We studied pancreases obtained at autopsy from individuals (n = 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls (n = 11).
In the men with prior transplant of female cord blood, there were 3.4 +/- 0.3% XX-positive insulin-expressing islet cells compared with 0.32 +/- 0.05% (p < 0.01) in male controls. In women with prior transplant of male cord blood cells we detected 1.03 +/- 0.20% XY insulin-expressing islet cells compared with 0.03 +/- 0.03 in female controls (p < 0. 001).
Cord blood stem cells have the capacity to differentiate into insulin-expressing cells in non-diabetic humans. It remains to be established whether these cells have the properties of beta cells.

• 出版日期2011-5