A novel amperometric immunosensor based on acetone-extracted propolis for the detection of the HIV-1 p24 antigen

作者：Kheiri F; Sabzi R E^*; Jannatdoust E; Shojaeefar E; Sedghi H

来源：Biosensors and Bioelectronics, 2011, 26(11): 4457-4463.

DOI：10.1016/j.bios.2011.05.002

• Immunosensor
• p24 antigen
• Gold nanoparticles
• Acetone-extracted propolis

A novel amperometric immunosensor for the detection of the p24 antigen (p24Ag) from HIV-1 was constructed using gold nanoparticles (GNP), multi-walled carbon nanotubes (MWCNTs), and an acetone-extracted propolis film (AEP). First, amino-functionalized MWCNTs (MWCNTNH(2)) were prepared and dispersed in an HAuCl(4) solution to synthesize GNPs in situ. Next, the GNP/CNT/AEP nanocomposite was prepared by mixing an AEP solution and the GNP/CNT powder. The nanocomposite was dripped onto a gold electrode (GE), and then p24 antibody (anti-p24 Ab) was immobilized on the resulting modified gold electrode to construct the immunosensor. The assembly process was characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors that were likely to influence the performance of the proposed immunosensor were studied in detail. Under optimal conditions, the proposed immunosensor exhibited good electrochemical sensitivity to the presence of p24 in a concentration range of 0.01 to 60.00 ng/mL, with a relatively low detection limit of 0.0064 ng/mL(S/N = 3). Moreover, the proposed immunosensor showed a rapid (<= 18 s) and highly sensitive amperometric response (0.018 and 1.940 mu A/ng mL(-1)) to p24 with acceptable stability and reproducibility.

• 出版日期2011-7-15