Hydrogen saline suppresses neuronal cell apoptosis and inhibits the p38 mitogen-activated protein kinase-caspase-3 signaling pathway following cerebral ischemia-reperfusion injury

作者：Li, Da; Ai, Yanqiu^*

来源：Molecular Medicine Reports, 2017, 16(4): 5321-5325.

DOI：10.3892/mmr.2017.7294

• hydrogen rich saline
• apoptosis
• p38 mitogen-activated protein kinase
• caspase-3
• ischemia-reperfusion injury

Cerebral ischemia-reperfusion injury (CIRI) is a serious pathological disease that is associated with a high rate death and disability. Saturated hydrogen (H-2) saline exhibits brain protective functions through anti-inflammatory, antioxidant and antiapoptotic effects. The present study investigated the potential treatment effects of H-2 on CIRI. In addition, the potential protective mechanisms of H-2 in the prevention of CIRI were investigated. Adult, male Sprague-Dawley rats (n=60) were randomly divided into the following three groups: Sham-operated group; IR group; and IR + H-2 group (0.6 mmol/l, 0.5 ml/kg/day). Hematoxylin and eosin, and TUNEL staining were performed for histopathological analysis and investigation of apoptosis, respectively. In addition, the protein expression of caspase-3, p38 mitogen-activated protein kinase (MAPK) and phosphorylated-p38 MAPK in the cortex were measured by western blotting analysis. These results demonstrated that H-2 significantly reduced the number of apoptotic cells, and the protein expression of p38 MAPK and caspase-3, compared with the IR group. These effects may be associated with the p38MAPK signaling pathway.

• 出版日期2017-10
• 单位郑州大学