Development of a Composite Chiral Stationary Phase from BSA and beta-Cyclodextrin-Bonded Silica

作者：Yao Bixia; Yang Xinmei; Guo Lizhen; Kang Shanshan; Weng Wen^*

来源：Journal of Chromatographic Science, 2014, 52(10): 1233-1238.

DOI：10.1093/chromsci/bmt203

A composite chiral stationary phase (CSP) derived from bovine serum albumin (BSA) and beta-cyclodextrin (CD)-bonded silica was prepared. 2,4,6-Trichloro-1,3,5-triazine was used as a cross-linker. The obtained CSP was applied to the enantioseparation of tryptophan, hydrobenzoin, phenylalanine and mandelic acid. The influences of eluent pH value, organic modifier and column temperature on the retention and enantioseparation were discussed. Tryptophan and hydrobenzoin achieved excellent resolution on the composite CSP. For tryptophan, the highest selectivity, 2.79, was achieved with 1% of methanol at pH 8.0. For hydrobenzoin, the selectivity could reach 1.42. The chromatographic results were compared with that on beta-CD-bonded or BSA-immobilized CSP.

• 出版日期2014-12
• 单位闽南师范大学