A standard cytogenetic photomap for the mosquito Anopheles stephensi (Diptera : Culicidae): Application for physical mapping

作者：Sharakhova Maria V; Xia Ai; McAlister Sarah I; Sharakhov Igor V^*

来源：Journal of Medical Entomology, 2006, 43(5): 861-866.

DOI：10.1603/0022-2585(2006)43[861:ASCPFT]2.0.CO;2

• Anopheles stephensi
• cytogenetic photomap
• polytene chromosomes
• fluorescent in situ hybridization

To facilitate physical genome mapping, we have developed a new cytogenetic photomap for Anopheles stephensi (Liston) (Diptera: Culicidae), an important malaria vector in Asia. The high-resolution images of the ovarian polytene chromosomes have been straightened and divided by numbered divisions and lettered subdivisions. The exact chromosomal locations of eight DNA probes have been determined by fluorescent in situ hybridization. Using the DNA sequences, we have established correspondence between chromosomal arms among An. stephensi, Anopheles gambiae (Patton), and Anopheles funestus (Giles). The results support previous cytogenetic observations of arm translocations taking place during diversification of the species. To make the cytogenetic map useful for population genetics studies, we have indicated the chromosomal positions for the breakpoints of 19 polymorpbic inversions.

• 出版日期2006-9
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)