Use of Vitamin K-deficient AIN-93 Diet is an Effective Approach to Create Subclinical Vitamin K Deficiency in C57BL/6 Mice

作者：Fu Xueyan^*; Smith D; Shen X; Booth S L

来源：Animal Nutrition and Feed Technology, 2014, 14(3): 421-429.

DOI：10.5958/0974-181X.2014.01345.6

• Bleeding
• Mice
• Phylloquinone
• Vitamin K-deficiency

Despite the popularity of genetically modified mouse models for mechanistic studies of human disease, little is known about the vitamin K requirements for mice. In the current study, we performed a small pilot time course study to determine the least amount of time required to reduce tissue concentrations of vitamin K without causing clinical signs of deficiency, such as abnormal bleeding. Female C57BL/6 mice were acclimated with AIN-93 G diet (903.5 +/- 26.5 mu g phylloquinone/kg diet) for 1 wk and subsequently fed a vitamin K-deficient AIN-93 G diet (21.4 +/- 3.0 mu g phylloquinone/kg diet) for 0d (0D group; n=3), 7d (7D group; n=3), 14d (14D group; n=3) and 28d (28D group; n=3) while limiting coprophagy. Phylloquinone and menaquinone-4 (MK-4) concentrations were measured in serum, liver, kidney, brain, pancreas, and body fat (omental). Compared to 0D group, there was a reduction of vitamin K concentrations in all analyzed tissues within 7d (P < 0.05), with the exception of body fat. In the 28D group, vitamin K was not detectable in mouse liver, yet there were no clinical signs of vitamin Kdeficiency, including bleeding. In summary, a vitamin K deficient diet can be used to induce low vitamin K tissue concentrations within 7d.

• 出版日期2014-9