APOBEC3B: A Potential Factor Suppressing Growth of Human Hepatocellular Carcinoma Cells

作者：Wu Pei Fung; Chen Yaw Sen; Kuo Ting Yin; Lin Hsi Hsun; Liu Ching Wen; Chang Li Ching^*

来源：Anticancer Research, 2015, 35(3): 1521-1527.

• Hepatocellular carcinoma (HCC)
• apolipoprotein B mRNA editing enzyme
• catalytic polypeptide-like 3B (APOBEC3B)
• vascular invasion
• Hep 3B

Background: To realize the role of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 38 (APOBEC3B) in hepatocellular carcinoma (HCC) occurrence, mRNAs of APOBEC3B from tumor and non-tumor tissues of patients with hepatectomy were isolated and in vitro studies were designed. Materials and Methods: Seventy-two tumor and non-tumor tissue samples, as well as clinical data, were collected from HCC patients during hepatectomy. The mRNA of APOBEC3B was assessed by real-time polymerase chain reaction. The viability of pLV-APOBEC3B-transfected Hep 38 cells was then determined. Cell growth of pLV-APOBEC3B-transfected Hep 38 cells was evaluated by in vitro migration assay. Results: The real-time polymerase chain reaction results indicated a higher expression of APOBEC3B mRNA in tumor tissues than in non-tumor tissues of patients with HBsAg(+) HCC. The expression of APOBEC3B in tumor or non-tumor tissue was not found to be a risk factor of recurrence in patients with HCC. The cell viability assay results indicated the growth-inhibitory effects of APOBEC3B on Hep 3B cells. The cell migration results indicated that APOBEC3B inhibits wound healing in Hep 3B cells. Conclusion: Based on these observations, we infer that APOBEC3B is a potential factor contributing to suppression of tumor growth in HCC.

• 出版日期2015-3