mRNA Expression Levels Among Cell Regulatory and DNA Damage Genes in Benzene-exposed Workers in China

作者：Wang Qi; Ye Rong; Ye Yun jie; Wan Jun xiang; Sun Pin; Zhu Yiliang; Au William; Xia Zhao lin^*

来源：Journal of Occupational and Environmental Medicine, 2012, 54(12): 1467-1470.

DOI：10.1097/JOM.0b013e318223d56c

Objective: We investigated how cells respond to the induction of DNA damage, focusing specifically on mRNA expression levels of cell regulatory and DNA repair genes under exposure to benzene. Method: The study sample was classified into three groups: direct exposure to benzene (A), indirect exposure to benzene (B), and non-exposed (C). Concentrations of benzene in the air of workplaces were monitored. Further blood biochemical parameters, cell cycle-regulated and DNA damage-related genes expression were analyzed. Results: The mRNA expression levels of Apel, Rad51, Bcl-2, Bax, Xpa, and Xpc genes were significantly down-regulated in groups A and B, with a dramatic up-regulation of p21 gene in group A accompanied by significantly lower counts of white blood cells, hemoglobin, platelets and lymphocyte subsets of CD8+, CD4+, T and B cells. Conclusion: The results indicated that exposure to benzene had significantly altered mRNA expression of some critical cell regulatory and DNA repair genes.

• 出版日期2012-12
• 单位汕头大学; 复旦大学; 杭州市疾病预防控制中心