A C-TERMINAL PROLINE IS REQUIRED FOR BIOLUMINESCENCE OF THE CA-2+-BINDING PHOTOPROTEIN, AEQUORIN

作者：NOMURA M; INOUYE S; OHMIYA Y; TSUJI FI

来源：FEBS Letters, 1991, 295(1-3): 63-66.

DOI：10.1016/0014-5793(91)81385-L

• AEQUORIN
• CA2+-BINDING PROTEIN
• BIOLUMINESCENCE
• CARBOXYL-TERMINUS
• PROLINE
• DELETION MUTANT

The requirement for a proline residue at the C-terminus of the Ca2+-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.

• 出版日期1991-12-16