Alternariol induce toxicity via cell death and mitochondrial damage on Caco-2 cells

作者：Fernandez Blanco Celia; Juan Garcia Ana; Juan Cristina; Font Guillermina; Ruiz Maria Jose

来源：Food and Chemical Toxicology, 2016, 88: 32-39.

DOI：10.1016/j.fct.2015.11.022

• Alternariol
• Cell cycle
• Membrane potential
• Necrosis-apoptosis
• Degradation products
• LC-MS/MS
• Caco-2 cells

Alternariol (AOH), a mycotoxin produced by Alternaria sp, appears as food contaminant in fruit, vegetables and cereal products. Its toxicity has been demonstrated, but the mechanisms involved have not been elucidated yet. In this study, the pathways triggered by AOH and degradation products generated on Caco-2 cells were evaluated. Cells were exposed to AOH sub-cytotoxic concentrations of 15, 30 and 60 mu M. Cell cycle disruption, the induction of apoptosis/necrosis and changes in mitochondrial membrane potential (Delta psi m) after 24 and 48 h was asses by flow cytometry. Also, AOH and its degradation products were evaluated after 24 and 48 h by high-performance liquid chromatography with tandem mass spectrometric (LC-MS/MS) to detect and quantify its levels. Cell cycle was significantly decreased at G1 phase and increased at S and G2/M phase at the time of exposure. AOH induced necrosis, apoptosis/necrosis and loss of Delta psi m in a dose and time-dependent manner. The concentrations of AOH quantified in the culture media exposed to AOH decreased as the exposure time was increased. In conclusion, AOH caused cytotoxic effects supported by blocking cell cycle, decreasing cell proliferation and increasing apoptosis/necrosis cells.

• 出版日期2016-2