科研之友
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摘要
Chlortetracycline (CTC) is the most frequently used antibacterial in today's swine production for disease treatment and growth promotion. It is not absorbed completely and a considerable amount is excreted into manure when CTC is administrated to animal. For this reason there is high concern on potential risks that manure-derived CTC enters into the surface water and selects for resistant microorganism. In order to support the monitoring of CTC and study its fate in manure and environment, a simple and rapid analytical procedure was developed to determine chlortetracycline (CTC) in pig solid manure. The extraction solution, composed of acetone-4mol.L(-1) hydrochloric acid-water (13: 1: 6, v/v/v), was mixed into the manure sample, and the solution was pH adjusted at 2.0-2.2 before extraction for 20 min. Chromatographic separation was carried out on a C(18) column at 375 nm with UV detector, using isocratic elution with oxalic acid-methanol-acetonitrile (72: 14: 14), v/v/v) as mobile phase at room temperature. Recoveries of CTC spiked samples at the levels of 1.0 mg.kg(-1), 2.0 mg.kg(-1) and 10.0 mg.kg(-1) were 92.2%-100.73% with relative standard deviation (RSD) of 2.81-7.11% intra-day and 1.343.25% inter-day, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.2 mg.kg(-1) and 0.8 mg.kg(-1), respectively. The procedure developed in this study was tested to be simple, sensitive and suitable for the determination and screening of CTC in pig solid manure samples.
