Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils

作者：Ruiz Perez Nancy J; Gonzalez Avila Marisela; Sanchez Navarrete Jaime; Toscano Garibay Julia D; Moreno Eutimio Mario A; Sandoval Hernandez Teresa; Arriaga Alba Myriam^*

来源：Scientific Reports, 2016, 6(1): 25371.

DOI：10.1038/srep25371

The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and a-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), beta-thujene (14.85%), beta-pinene (12.79%) and alpha-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 mu g respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42-3.71 mu g and for C. latifolia of 0.22-1.30 mu g. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 mu g. These properties might bestow different odontological applications to each essential oil.

• 出版日期2016-5-3