Different Selected Mechanisms Attenuated the Inhibitory Interaction of KIR2DL1 with C2(+) HLA-C in Two Indigenous Human Populations in Southern Africa

作者：Nemat Gorgani Neda; Hilton Hugo G; Henn Brenna M; Lin Meng; Gignoux Christopher R; Myrick Justin W; Werely Cedric J; Granka Julie M; Moeller Mario; Hoa Eileen G; Yawata Makoto; Yawata Nobuyo; Boelen Lies; Asquith Becca; Parham Peter; Norman Paul J

来源：The Journal of Immunology, 2018, 200(8): 2640-2655.

DOI：10.4049/jimmunol.1701780

The functions of human NK cells in defense against pathogens and placental development during reproduction are modulated by interactions of killer cell Ig-like receptors (KIRs) with HLA-A, -B and-C class I ligands. Both receptors and ligands are highly polymorphic and exhibit extensive differences between human populations. Indigenous to southern Africa are the KhoeSan, the most ancient group of modern human populations, who have highest genomic diversity worldwide. We studied two KhoeSan populations, the Nama pastoralists and the not equal Khomani San hunter-gatherers. Comprehensive next-generation sequence analysis of HLA-A,-B, and-C and all KIR genes identified 248 different KIR and 137 HLA class I, which assort into similar to 200 haplotypes for each gene family. All 74 Nama and 78 not equal Khomani San studied have different genotypes. Numerous novel KIR alleles were identified, including three arising by intergenic recombination. On average, KhoeSan individuals have seven to eight pairs of interacting KIR and HLA class I ligands, the highest diversity and divergence of polymorphic NK cell receptors and ligands observed to date. In this context of high genetic diversity, both the Nama and the not equal Khomani San have an unusually conserved, centromeric KIR haplotype that has arisen to high frequency and is different in the two KhoeSan populations. Distinguishing these haplotypes are independent mutations in KIR2DL1, which both prevent KIR2DL1 from functioning as an inhibitory receptor for C2+ HLA-C. The relatively high frequency of C2+ HLA-C in the Nama and the not equal Khomani San appears to have led to natural selection against strong inhibitory C2-specific KIR.

• 出版日期2018-4-15