Association of Deoxyhypusine Hydroxylase with Peroxiredoxin I was Modulated in the Yeast Saccharomyces cerevisiae under Oxidative Stress

作者：Shim Doobo; Park Eun Sil; Sim Gyujin; Lee Ji Young; Kang Ju Hwan; Yoo Hyun Joo; Choi Yeon Jin; Lee Young Mee; Lee Sang Yeol; Kim Min Gab; Kang Dawon; Jung Eun Jung; Kang Kee Ryeon^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2011, 54(4): 515-523.

DOI：10.3839/jksabc.2011.079

• deoxyhypusine hydroxylase
• oxidative stress
• peroxiredoxin I
• proteomics
• redox state

Eukaryotic translation initiation factor 5A (eIF5A) is the only hypusine-containing protein, which is formed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). DOH H is a novel metalloenzyme with HEAT vertical bar named for human huntingtin (H), elongation factor 3 (E), a subunit of protein phosphatase 2A (A), and the target of rapamycin (T)vertical bar-repeat motifs. Inspite of much progress in determining the roles of iron-containing DOHH holoenzyme as an eIF5A hydroxylase, little is known about iron-free apoenzyme of DOHH under certain stress conditions. For this purpose, we compared cell growth in two yeast strains subjected to oxidative damage. Thus, the existence of more viable cells in the Saccharomyces cerevisiae BY4743 (parental yeast) strain than in the DOH H-null strain after H(2)O(2) treatment indicates the importance of DOHH in protecting yeast cells against oxidative stress. To identify endogenous target proteins influenced by DOHH under oxidative damage, proteomic analysis was applied to the two yeast strains. Of these proteins, the oxidized form of peroxiredoxin I (PrxI) was concomitantly up-regulated in both strains under H(2)O(2) treatment. Two-dimensional electrophoresis (DE) followed by immunoblot analysis shows that the recovery of the oxidized PrxI to the reduced enzyme under H(2)O(2) treatment was found to be much slower in the DOHH-null strain than in the parental strain. Based on the results, we discovered a possible interaction between DOHH and PrxI by immunoprecipitation/immunoblotting in yeast under oxidative stress. Taken together, these results suggest that DOHH might be a candidate protein for protection of yeast cells against oxidative stress in conjunction with PrxI.

• 出版日期2011-8