Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China

作者：Gao Shenyang; Li Dandan; Fan Chen; Wu Shuliang; Zhou Tiezhong^*

来源：Archives of Virology, 2011, 156(9): 1621-1626.

DOI：10.1007/s00705-011-1007-y

• Hepatitis E virus
• Epitope mapping
• ELISA
• Serological surveillance

In this study, seven recombinant epitope peptides from within the ORF2 protein of the local genotype 4 swine hepatitis E virus (HEV) DQ strain were designed and analyzed. Then, a new multi-epitope-based ELISA was established. In comparison with a commercial kit, this test exhibited good specificity and sensitivity for anti-HEV genotype 4. Subsequently, this test was applied for analyzing serum samples from either swine herds or human populations in northern China. The overall seroprevalence rate of anti-HEV IgG reached up to 40.4% for swine and 8.1% for humans. A statistical difference was observed for humans in rural and urban areas, with a higher prevalence for people living in rural than urban areas. Moreover, sequencing confirmed that all RNA-positive samples belonged to genotype 4.

• 出版日期2011-9
• 单位聊城大学; 锦州医科大学; 海南出入境检验检疫局检验检疫技术中心