科研之友
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摘要
In present work, the analysis method of semen euphorbiae frostlike powders with HPLC fingerprint was established. The high performance liquid chromatography was applied. The chromatographic column was Agilent Zorbax-C-18 (4.6 x 250 mm, 5 mu m) column. The flow phase was acetonitrile: water = 61: 39; the flow velocity was: 1 mL min(-1), the detection wavelength was 280 nm and the column temperature was 40 degrees C. The fingerprints of semen euphorbiae frostlike powders were established and 7 common peaks were identified. Fingerprint of semen euphorbiae frostlike powder samples and that of the control group shared a similarity of at least 0.9 and can be used in the qualitative identification of semen euphorbiae frostlike powders.
