A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution

作者：Yap Thai Leong; Chen Yen Liang; Xu Ting; Wen Daying; Vasudevan Subhash G; Lescar Julien^*

来源：Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2007, 63: 78-83.

DOI：10.1107/S1744309106055084

Dengue virus, a member of the Flaviviridae genus, causes dengue fever, an important emerging disease with several million infections occurring annually for which no effective therapy exists. The viral RNA-dependent RNA polymerase NS5 plays an important role in virus replication and represents an interesting target for the development of specific antiviral compounds. Crystals that diffract to 1.85 angstrom resolution that are suitable for three-dimensional structure determination and thus for a structure-based drug-design program have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration.

• 出版日期2007-2
• 单位南阳理工学院