Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection

作者：Angeles Orellana Miguel Ma; Alcolea Medina Adela; Barrado Blanco Laura; Rodriguez Otero Joaquin; Chaves Sanchez Fernando

来源：Enfermedades Infecciosas y Microbiologia Clinica, 2013, 31(2): 97-99.

DOI：10.1016/j.eimc.2012.01.003

• Clostridium difficile infection
• Nosocomial diarrhoea
• Toxigenic Clostridium difficile

Objective: To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C difficile infection (CDI) based on this test.
Methods: We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.
Results: This test enabled us to obtain a conclusive result in <30 min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C dfficile by toxigenic culture.
Conclusion: This test has a high sensitivity and specificity for the diagnosis of CDI.

• 出版日期2013-2