Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein

作者：Schnell Jaimie A; Han Shuyou; Miki Brian L; Johnson Douglas A^*

来源：Plant Cell Reports, 2010, 29(9): 987-996.

DOI：10.1007/s00299-010-0884-y

• Soybean peroxidase
• N-terminal propeptide
• C-terminal propeptide
• Signal peptide
• Vacuole
• Seed coat

Elements that contribute to the high, stable yield of soybean peroxidase (SBP) in soybean seed coats can be exploited in the development of this tissue as a protein production platform. SBP contains an N-terminal and a C-terminal propeptide that are predicted to direct vacuolar targeting; this may be one factor that contributes to its high yield and stability. We characterized the function of the SBP propeptides and investigated their ability to increase the yield of a foreign protein in a heterologous plant system. SBP propeptides are functional signal peptides capable of directing vacuolar transport in Arabidopsis. The use of these propeptides as well as an endoplasmic reticulum (ER)-retention signal to direct a foreign protein to the apoplast, ER, or vacuole can significantly increase yield and will therefore be useful for the development of the seed coat as a protein production platform. We also demonstrate that growth conditions may have a significant impact on the yield of a foreign protein and that this may be subcellular compartment-specific.

• 出版日期2010-9