Antimicrobial and Anti-Inflammatory Activity of Chitosan Alginate Nanoparticles: A Targeted Therapy for Cutaneous Pathogens

作者：Friedman Adam J; Jenny Phan; Schairer David O; Champer Jackson; Qin Min; Pirouz Aslan; Blecher Paz Karin; Oren Ami; Liu Phil T; Modlin Robert L; Kim Jenny^*

来源：Journal of Investigative Dermatology, 2013, 133(5): 1231-1239.

DOI：10.1038/jid.2012.399

Advances in nanotechnology have demonstrated potential application of nanoparticles (NPs) for effective and targeted drug delivery. Here we investigated the antimicrobial and immunological properties and the feasibility of using NPs to deliver antimicrobial agents to treat a cutaneous pathogen. NPs synthesized with chitosan and alginate demonstrated a direct antimicrobial activity in vitro against Propionibacterium acnes, the bacterium linked to the pathogenesis of acne. By electron microscopy (EM) imaging, chitosan alginate NPs were found to induce the disruption of the P. acnes cell membrane, providing a mechanism for the bactericidal effect. The chitosan alginate NPs also exhibited anti-inflammatory properties as they inhibited P. acnes-induced inflammatory cytokine production in human monocytes and keratinocytes. Furthermore, benzoyl peroxide (BP), a commonly used antiacne drug, was effectively encapsulated in the chitosan alginate NPs and demonstrated superior antimicrobial activity against P. acnes compared with BP alone while demonstrating less toxicity to eukaryotic cells. Together, these data suggest the potential utility of topical delivery of chitosan alginate NPencapsulated drug therapy for the treatment of dermatologic conditions with infectious and inflammatory components.

• 出版日期2013-5