科研之友
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摘要
A convenient and sensitive fluorescence biosensor for carbamat pesticides was developed. By establishment of the genomic library of carbamat pesticides degrading bacteria H5, the regulatory sequence of carbamat pesticide responding gene was screened, which was then linked to gene sequence of green fluorescent protein to fabricate the cell. free fluorescence biosensor H12-E. The protein concentration of the cell. free system was 1.0 g/L. The carbofuran standards with different concentrations were detected at room temperature, and the results showed that the fg level of carbamat pesticides could be detected within 30 min at room temperature. The sensitivity was above the national standard, and the linear range was 1 ng/L-100 mu g/L. The water samples of Songhua River in Jilin province was collected and detected carbamate pesticide residues. Three levels of carbofuran were spiked into samples of Yitong River extract. The overall recoveries and the relative standard deviations (RSDs) were in the range of 95%-110% and 2.4%-4.9%. This assay proved to be a suitable tool for fast detection of carbamat pesticides residue in environmental water samples.
- 出版日期2014
- 单位长春理工大学
