Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes

作者：Liu Jifeng; Su Bin; Lagger Gregoire; Tacchini Philippe; Girault Hubert H^*

来源：Analytical Chemistry, 2006, 78(19): 6879-6884.

DOI：10.1021/ac0608624

Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double-strand DNA was adsorbed. A redox mediator, namely, tris-2,2';-bipyridine ruthenium(II) [Ru(bpy)(3)(2+)] was electrooxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solution. The resulting oxidation damage of the adsorbed dsDNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)(3)Cl-2 at a low concentration (mu M). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.

• 出版日期2006-10-1