A Dlx2-and Pax6-dependent transcriptional code for periglomerular neuron specification in the adult olfactory bulb

作者：Brill Monika S; Snapyan Marina; Wohlfrom Hilde; Ninkovic Jovica; Jawerka Melanie; Mastick Grant S; Ashery Padan Ruth; Saghatelyan Armen; Berninger Benedikt; Goetz Magdalena

来源：Journal of Neuroscience, 2008, 28(25): 6439-6452.

DOI：10.1523/JNEUROSCI.0700-08.2008

• neurogenesis
• subependymal zone
• olfactory bulb
• transcription factor
• tyrosine hydroxylase
• stem cell

Distinct olfactory bulb (OB) interneurons are thought to become specified depending on from which of the different subregions lining the lateral ventricle wall they originate, but the role of region-specific transcription factors (TFs) in the generation of OB interneurons diversity is still poorly understood. Despite the crucial roles of the Dlx family of TFs for patterning and neurogenesis in the ventral telencephalon during embryonic development, their role in adult neurogenesis has not yet been addressed. Here we show that in the adult brain, Dlx 1 and Dlx2 are expressed in progenitors of the lateral but not the dorsal subependymal zone (SEZ), thus exhibiting a striking regional specificity. Using retroviral vectors to examine the function of Dlx2 in a cell-autonomous manner, we demonstrate that this TF is necessary for neurogenesis of virtually all OB interneurons arising from the lateral SEZ. Beyond its function in generic neurogenesis, Dlx2 also plays a crucial role in neuronal subtype specification in the OB, promoting specification of adult-born periglomerular neurons (PGNs) toward a dopaminergic fate. Strikingly, Dlx2 requires interaction with Pax6, because Pax6 deletion blocks Dlx2-mediated PGN specification. Thus, Dlx2 wields a dual function by first instructing generic neurogenesis from adult precursors and subsequently specifying PGN subtypes in conjunction with Pax6.

• 出版日期2008-6-18