An improved method for cell-to-cell transmission of infectious prion

作者：Tanaka Masahiko; Hara Hideyuki; Nishina Hiroshi; Hanada Kentaro; Hagiwara Ken'ichi^*; Maehama Tomohiko

来源：Biochemical and Biophysical Research Communications, 2010, 397(3): 505-508.

DOI：10.1016/j.bbrc.2010.05.143

• Prion
• Transmission
• Co-culture method

Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP(Sc)), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP(Sc) transmission from ScN2a cell, which persistently retains PrP(Sc), to naive N2a cell. In this cell-to-cell transmission system, PrP(Sc) transmitted to recipient N2a cell was able to be detected within 5-7 days. Further characterization showed that higher cell density greatly facilitated the transmission of PrP(Sc). This improved in vitro transmission method may become a useful tool for unveiling the molecular mechanism of PrP(Sc) transmission.

• 出版日期2010-7-2