科研之友
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摘要
Background: The aim of this study was to assess mRNA of IL-6, TNF alpha and IL-10 cytokines in bone marrow, possible mediators involved in altered bone remodeling with detrimental consequences on bone quality in NGR (Nutritional growth retardation) rats. Methods: Weanling male Wistar rats were assigned either to control (C) or experimental group (NGR) (n = 20 each). C and NGR groups were assigned to 2 groups according to receiving saline solution (SS) or propranolol hydrochloride (P): C, C + P (CP), NGR or NGR + P (NGRP). For 4 weeks, NGR and NGRP rats received 80% of the amount of food consumed by C and CP, respectively, the previous day, corrected by body weight. P (7 mg/kg/day) was injected ip 5 days/week, for 4 weeks in CP and NGRP rats. Body weight and length were recorded. After 4 weeks, blood was drawn. Femurs were dissected for RNA isolation from bone marrow and mRNA of cytokines assays. Results: Food restriction induced a significant negative effect on body growth in NGR and NGRP rats (p < 0.001). P had no effects on zoometric parameters (p > 0.05). CTX-I increased in NGR rats vs. C (p < 0.001), but diminished in NGRP (p < 0.01). Serum osteocalcin, PTH, calcium and phosphate levels remained unchanged between groups (p > 0.05). In NGR, bone marrow IL-6 mRNA and IL-10 mRNA levels were low as compared to other groups (p < 0.05). In contrast, bone marrow TNF-alpha mRNA levels were significantly high (p < 0.05). Conclusions: This study provides evidences that NGR outcomes in a bone marrow proinflammatory microenvironment leading to unbalanced bone remodeling by enhancement of bone resorption reverted by propranolol.
- 出版日期2014-10
