Application of Probe Electrospray Ionization Mass Spectrometry (PESI-MS) to Clinical Diagnosis: Solvent Effect on Lipid Analysis

作者：Mandal Mridul Kanti; Yoshimura Kentaro; Chen Lee Chuin; Yu Zhan; Nakazawa Tadao; Katoh Ryohei; Fujii Hideki; Takeda Sen; Nonami Hiroshi; Hiraoka Kenzo^*

来源：Journal of the American Society for Mass Spectrometry, 2012, 23(11): 2043-2047.

DOI：10.1007/s13361-012-0462-3

• Electrospray
• Probe Electrospray
• Pathology
• Biological Specimen
• Diagnostics

We have examined several combinations of solvents with the aim of optimizing the ionization conditions for molecular diagnosis of malignant tumours by PESI-MS. Although the best conditions may depend on the actual species in the sample, the optimal conditions for renal cell carcinoma (RCC) were achieved by using alcohols. PESI-MS successfully delineated the differential expression of phospholipids (PCs) and triacylglycerols (TAGs) in noncancerous and RCC tissues by using these solvent systems. This study paves the way for the application of PESI-MS in medical samples.

• 出版日期2012-11
• 单位沈阳师范大学