Gold nanoparticles modified electrode via simple electrografting of in situ generated mercaptophenyl diazonium cations for development of DNA electrochemical biosensor

作者：Li, Feng; Feng, Yan; Dong, Pingjun; Yang, Limin; Tang, Bo^*

来源：Biosensors and Bioelectronics, 2011, 26(5): 1947-1952.

DOI：10.1016/j.bios.2010.07.076

• Biosensor
• DNA
• Gold nanoparticles
• Electrografting
• Mercaptophenyl

A novel protocol for development of DNA electrochemical biosensor based on gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE) was proposed, which was carried out by the self-assembly of AuNPs on the mercaptophenyl film (MPF) via simple electrografting of in situ generated mercaptophenyl diazonium cations. The resulting MPF was covalently immobilized on GCE surface via C-C bond with high stability, which was desirable in fabrication of excellent performance biosensors. Probe DNA was self-assembled on AuNPs through the well-known Au-thiol binding. The recognition of fabricated DNA electrochemical biosensor toward complementary single-stranded DNA was determined by differential pulse voltammetry with the use of Co(phen)(3)(3+) as the electrochemical indicator. Taking advantage of amplification effects of AuNPs and stability of MPF, the developed biosensor could detect target DNA with the detection limit of 7.2 x 10(-11) M, which also exhibits good selectivity, stability and regeneration ability for DNA detection.

• 出版日期2011-1-15
• 单位山东师范大学; 青岛科技大学