Application of a europium complex, Eu(AA)(3)phen (AA = acrylic acid, phen=1,10-phenanthroline) as a spectroscopic probe and cleaving reagent of DNA

作者：Li Ling; Pan Quan; Song Gong Wu^*

来源：Materials Science and Engineering: C , 2013, 33(4): 2078-2083.

DOI：10.1016/j.msec.2013.01.027

• DNA
• Eu(AA)(3)phen complex
• Interaction
• Electrostatic binding

A complex Eu(AA)(3)phen was synthesized and characterized by elemental analysis, IR spectroscopy and H-1 NMR. Interaction between Eu(AA)(3)phen and DNA was studied by UV/visible absorption spectroscopy, fluorescence spectrophotometer, circular-dichroism (CD) spectra and gel-electrophoresis measurements. Absorption spectral indicated that Eu(AA)(3)phen binding to DNA was an electrostatic mode, which was authenticated by the effect of ionic strength, thermal denaturation and fluorescence quenching experiments. The intrinsic binding constant K-b of Eu(AA)(3)phen was calculated to be 1.6 x 10(4) L mol(-1).

• 出版日期2013-5-1
• 单位湖北大学