Absence of CIP1/KIP1 Hypermethylation in Gastric Cancer Patients from Northern Brazil

作者：Borges Barbara Do Nascimento^*; Rodriguez Burbano Rommel Mario; Harada Maria Lucia

来源：In Vivo, 2010, 24(4): 579-582.

• Methylation
• P21(CIP1)
• P27(KIP1)
• immunohistochemistry
• gastric cancer
• Brazil

The aim of this study was to investigate the protein expression and methylation pattern of P21(CIP1) and P27(KIP1) genes. Patients and Methods: Twenty samples of gastric tumor and non-tumoral tissues of patients from Para state, Brazil were collected. Methylation patterns were assessed by bisulfite sequencing, and protein expression evaluated with immunohistochemical analysis. Results: None of the analyzed samples showed methylation in both genes. Immuno-histochemistry analysis demonstrated mislocalization or absence of expression of P21(CIP1) and P27(KIP1) in 7/20 and 6/20 of the studied samples, respectively. No correlations regarding protein expression and clinicopathological characteristics were observed; down-regulation of expression of P21(CIP1) with low (I-II) tumor stage (p=0.0777), and older age (>50 years old) with negative or mislocalization of P27(KIP1) (p=0.0922) were of borderline the statistical significance. Conclusion: Our results suggest that hypermethylation does not contribute to P21(CIP1) and P27(KIP1) silencing in gastric cancer, and that the role of these genes in the gastric tumorigenesis pathways should be studied further in the Para state population.

• 出版日期2010-8