Aging changes the chromatin configuration and histone methylation of mouse oocytes at germinal vesicle stage

作者：Manosalva I^*; Gonzalez A

来源：Theriogenology, 2010, 74(9): 1539-1547.

DOI：10.1016/j.theriogenology.2010.06.024

• Aging
• Cbx 1
• Germinal vesicle
• Histone methylation
• Oocyte

Aging decreases the fertility of mammalian females In old oocytes at metaphase II stage (ME) there are alterations of the chromatin configuration and chromatin modifications such as histone acetylation Recent data indicate that alterations of histone acetylation at MII initially arise at germinal vesicle stage (GV) Therefore we hypothesized that the chromatin configuration and histone methylation could also change in old GV oocytes In agreement with our hypothesis young GV oocytes had non surrounded nucleolus (NSN) and surrounded nucleolus (SN) chromatin configurations, while old GV oocytes also had chromatin configurations that could not be classified as NSN or SN Regarding histone methylation, young GV and MII oocytes showed dimethylation of lysines 4, 9, 36 and 79 in histone 3 (H3K4me2, H3K9me2 H3K36me2 H3K79me2) lysine 20 in histone H4 (H4K20me2) and trimethylation of lysine 9 in histone 3 (H3K9me3) while a significant percentage of old GV and MII oocytes lacked H3K9me3 H3K36me2 H3K79me2 and H4K20me2 The percentage of old oocytes lacking histone methylation was similar at GV and MII suggesting that alterations of histone methylation in old MII oocytes initially arise at GV Besides the expression of the histone methylation-related factors Cbx 1 and Sirt 1 was also found to change in old GV oocytes In conclusion our study reports changes

• 出版日期2010-12