Aceriphyllum rossii Extract and Its Active Compounds, Quercetin and Kaempferol Inhibit IgE-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis

作者：Kim Myungsuk; Lim Sue Ji; Kang Suk Woo; Um Byung Hun; Nho Chu Won^*

来源：Journal of Agricultural and Food Chemistry, 2014, 62(17): 3750-3758.

DOI：10.1021/jf405486c

• quercetin
• kaempferol
• Aceriphyllum rossii
• IgE-mediated allergic diseases
• RBL-2H3 mast cells

Aceriphyllum rossii contains an abundant source of natural flavonoids with potential antioxidant, anticancer and anti-inflammatory properties. However, the effect of A. rossii extract (ARE) on immunoglobulin E(IgE)-mediated allergic responses remains unknown. In the present study, the effects of ARE and its active compounds, quercetin and kaempferol, on IgE-mediated rat basophilic leukemia mast cell activation and passive cutaneous anaphylaxis (PCA) were investigated. ARE, quercetin, and kaempferol inhibited secretion of beta-hexosaminidase and histamine, and reduced the production and mRNA expression of interleukin-4 and tumor necrosis factor-a. ARE also decreased the production of prostaglandin E-2 and leukotriene B, and expression of cyclooxygenase 2 and 5-lipoxygenase. Furthermore, ARE, quercetin, and kaempferol inhibited IgE-mediated phosphorylation of Syk, phospholipase C gamma, protein kinase C (PKC)mu, and the mitogen-activated protein kinases, extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. ARE, quercetin, and kaempferol markedly suppressed mast cell-dependent PCA in IgE-sensitized mice. These results indicate that ARE and its active constituents, quercetin and kaempferol, may be a useful therapy for immediate-type hypersensitivity.

• 出版日期2014-4-30