Assessing the impacts of total liquid ventilation on left ventricular diastolic function in a model of neonatal respiratory distress syndrome

作者：Sage Michael; Nadeau Mathieu; Forand Choiniere Claudia; Mousseau Julien; Vandamme Jonathan; Berger Claire; Tremblay Roy Jean Sebastien; Tissier Renaud; Micheau Philippe; Fortin Pellerin Etienne^*

来源：PLos One, 2018, 13(1): e0191885.

DOI：10.1371/journal.pone.0191885

Background
Filling the lung with dense liquid perfluorocarbons during total liquid ventilation (TLV) might compress the myocardium, a plausible explanation for the instability occasionally reported with this technique. Our objective is to assess the impacts of TLV on the cardiovascular system, particularly left ventricular diastolic function, in an ovine model of neonatal respiratory distress syndrome.
Method
Eight newborns lambs, 3.0 +/- 0.4 days (3.2 +/- 0.3kg) were used in this crossover experimental study. Animals were intubated, anesthetized and paralyzed. Catheters were inserted in the femoral and pulmonary arteries. A high-fidelity pressure catheter was inserted into the left ventricle. Surfactant deficiency was induced by repeated lung lavages with normal saline. TLV was then conducted for 2 hours using a liquid ventilator prototype. Thoracic echocardiography and cardiac output assessment by thermodilution were performed before and during TLV.
Results
Left ventricular end diastolic pressure (LVEDP) (9.3 +/- 2.1 vs. 9.2 +/- 2.4mmHg, p = 0.89) and dimension (1.90 +/- 0.09 vs. 1.86 +/- 0.12cm, p = 0.72), negative dP/dt (-2589 +/- 691 vs. -3115 +/- 866mmHg/s, p = 0.50) and cardiac output (436 +/- 28 vs. 481 +/- 59ml/kg/min, p = 0.26) were not affected by TLV initiation. Left ventricular relaxation time constant (tau) slightly increased from 21.5 +/- 3.3 to 24.9 +/- 3.7ms (p = 0.03). Mean arterial systemic (48 +/- 6 vs. 53 +/- 7mmHg, p = 0.38) and pulmonary pressures (31.3 +/- 2.5 vs. 30.4 +/- 2.3mmHg, p= 0.61) were stable. As expected, the inspiratory phase of liquid cycling exhibited a small but significant effect on most variables (i.e. central venous pressure +2.6 +/- 0.5mmHg, p = 0.001; LVEDP +1.18 +/- 0.12mmHg, p<0.001).
Conclusions
TLV was well tolerated in our neonatal lamb model of severe respiratory distress syndrome and had limited impact on left ventricle diastolic function when compared to conventional mechanical ventilation.

• 出版日期2018-1-29