Assessment of genetic diversity using SSR and candidate genes markers among rice accessions (Orysa sativa L.) in relation to grain micronutrient (Iron and Zinc) content

作者：Suma M R^*; Shashidhar H E

来源：Research Journal of Biotechnology, 2015, 10(3): 37-43.

• SSR
• CG
• genetic diversity
• Orepa sativa L.

Assessment of genetic diversity in a crop germplasm is a vital part of plant breeding. DNA markers such as microsatellite or simple sequence repeat markers (SSR) and candidate genes (CG) have been widely used to estimate the genetic diversity in rice. Out of 50 rice SSR primer and CGs pairs studied, 47 showed polymorphism on screening in 12 rice genotypes. The trend of diversity was estimated based on the number of alleles (Na), Nei's genetic diversity index (He) and polymorphism information content (PIC). A total of 141 alleles were identified averaging 2.82 alleles per locus. PIC varied from 0.24 (RM5707) to 0.71 (RM1089 and RM264). The Nei's genetic diversity (he) varied from 0.28 (RM5707) to 0. 68 (RM144), the average number of he value estimated in present study is 0.53. The NTSYS-pc UPGMA tree cluster analysis led to the distribution of twelve rice varieties into two major groups, I and II. Azucena and IR64 had minimum similarity coefficient of 0.122. Similarity coefficient values indicated that Azucena and IR64 are highly diverse which may be used in further breeding programme for crop improvement.

• 出版日期2015-3