Antioxidant activity of newly discovered lineage of marine actinobacteria

作者：Karthik Loganathan; Kumar Gaurav; Rao Kokati Venkata Bhaskara^*

来源：Asian Pacific Journal of Tropical Medicine, 2013, 6(4): 325-332.

DOI：10.1016/S1995-7645(13)60065-6

• Streptornyces LK-3
• Antioxidant
• DPPH radical scavenging assay
• Cytotoxicity

Objective: To investigate the antioxidant activity of marine actinobacteria. Methods: The content of total phenolics, the level of antioxidant potential by DPPH radical scavenging activity, metal chelating activity, FRAP method, beta carotene assay and NO scavenging activity in extract were determined. Results: In all the methods the extract exhibited good scavenging activity except NO scavenging activity. The IC50 values of marine actinobacteria extract on DPPH radical were found to he 41.09 mu g/mL. The zone of color retention was 12 mm in beta -carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV- photolysed H2O2-driven oxidative damage to pBR322. HPLC analysis identified souse of the major phenolic compounds in extracts, which might be responsible for the antioxidant potential and cyto-protection. It showed a 100% cytotoxic effect in brine shrimp lethality assay within 10 mins. The novel actinobacteria was identified as Streptomyces LK-3 (JF710608) through 16S rDNA Sequencing. Conclusions: The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.

• 出版日期2013-4