摘要
Both glutathione capped CdTe quantum dots (QDs) and enzymes were encapsulated with poly(diallyldimethylammonium chloride) (PDDA) via electrostatic attraction to form hybrid films. The obtained PDDA QD-enzyme hybrids feature both high fluorescence and biorecognition. In the obtained hybrid materials, the fluorescence emission of the QDs was stable for at least 3 months, and the structure and activity of the enzyme was also well maintained as the Michaelis constant of tyrosinase was determined to be 0.90 mmol/L, which is just 2 times higher than that of free enzyme. This hybrid material was then utilized as a platform for the development of biosensors based on the quenching effects of the enzymatic products on the emission of the QDs with a kind of phenol (catechol) and glucose as example analytes. The detection limits of catechol and glucose were 1.0 x 10(-5) and 5.0 x 10(-6) mol/L, respectively. Moreover, this hybrid material was applied to the fabrication of test paper for these two analytes. The test paper was very stable with respect to the fluorescence of the QDs and the activity of the enzyme maintained for at least 1 month.
- 出版日期2012-6-5