Development of Schemes of Induced Mutagenesis for Improving the Productivity of Aspergillus Strains Producing Amylolytic Enzymes

作者：Kostyleva E V; Sereda A S; Velikoretskaya I A; Burtseva E I; Veselkina T N; Nefedova L I; Sharikov A Yu; Tsurikova N V; Lobanov N S; Sinitsyn A P

来源：Microbiology, 2017, 86(4): 493-502.

DOI：10.1134/S0026261717040087

• mutagenesis
• UV-irradiation
• gamma irradiation
• nitrosoguanidine
• Aspergillus awamori
• Aspergillus oryzae
• alpha-amylase
• glucoamylase

In order to improve the biosynthesis of amylolytic enzymes by industrial Aspergillus strains, the efficiency of stepwise application of the following methods of induced mutagenesis was studied: ultraviolet (UV) irradiation, gamma irradiation, and treatment with N-methyl-N-nitro-N-nitrosoguanidine (NG). It was found that at the early stages of mutagenesis of the glucoamylase-producing A. awamori strain UV and NG, used either alone or in combination, were efficient enough as mutagenic agents, providing an increase of glucoamylase activity by 30-35 and 50-60%, respectively. At the later stages, serial UV mutagenesis and gamma-irradiation showed high efficiency for both A. awamori, and A. oryzae strains. Gamma-mutagenesis of Aspergillus strains using a cobalt source provided the most stable and highly active strains retaining 90-95% of their activity after five transfers on agar medium. The experiments resulted in significant improvement of the studied industrial strains, more than doubling activity of the target enzymes.

• 出版日期2017-7