Analytical methods for the detection of viruses in food by example of CCL-3 bioagents

作者：Hartmann E M; Halden R U^*

来源：Analytical and Bioanalytical Chemistry, 2012, 404(9): 2527-2537.

DOI：10.1007/s00216-012-5974-y

• Norovirus
• Hepatitis A
• Adenovirus
• Enterovirus
• Processing
• Detection

This critical review presents challenges and strategies in the detection of viral contaminants in food products. Adenovirus, caliciviruses, enteroviruses, and hepatitis A are emerging contaminant viruses. These viruses contaminate a variety of food products, including fruits, vegetables, shellfish, and ready-to-eat processed foods. The diversity of targets and sample matrices presents unique challenges to virus monitoring that have been addressed by a wide array of processing and detection methods. This review covers sample acquisition and handling, virus recovery/concentration, and the determination of targets using molecular biology and mass-spectrometric approaches. The concentration methods discussed include precipitation, antibody-based concentration, and filtration; the detection methods discussed include microscopy, polymerase chain reaction, nucleic acid sequence-based amplification, and mass spectrometry.

• 出版日期2012-11