A reliable method for the selection and confirmation of transconjugants of plant growth-promoting bacteria especially plant-associated Burkholderia spp.

作者：Tariq Mohsin; Lum Michelle R; Chong Allan W; Amirapu Anjana B; Hameed Sohail; Hirsch Ann M^*

来源：Journal of Microbiological Methods, 2015, 117: 49-53.

DOI：10.1016/j.mimet.2015.07.008

• Conjugation
• Congo Red
• PGPB
• PCAT
• Transconjugants

Selectable markers, e.g., antibiotic resistance, for conjugation experiments are not always effective for slowgrowing plant growth promoting bacteria such as Burkholderia. We used PCAT medium containing Congo Red for selecting Burkholderia transconjugants. This method allows for the reliable selection of transconjugants of these novel plant growth-promoting bacteria.

• 出版日期2015-10