摘要

To investigate the effect of Maillard reaction (MR) and gastrointestinal digestion (GID) on the antihyperuricemic activity of tuna protein hydrolysate (TPH), hyperuricemia rats were treated with TPH, its MR products (TPH-M) and gastrointestinal digesta (TPH-D) in this work. Although the XO inhibitory activity of TPH-M was higher than TPH, TPH-M exhibited even lower serum-uric-acid-lowering activity than TPH. Additionally, there was no significant (P < 0.05) difference observed in the inhibition of XO activity in vitro and antihyperuricemic activity of TPH and TPH-D, indicated that the antihyperuricemic activity of TPH would not decrease after GID. Additionally, TPH, TPH-M and TPH-D could effectively inhibit XO activity and downregulate XO mRNA expression in vivo, suggesting the mechanisms of antihyperuricemic effect was related to XO inhibitory activity and XO mRNA expression. As a whole, TPH could be a useful functional ingredient against hyperuricemia.