摘要
The present study investigated the underlying molecular mechanism by which Buthus martensii Karsch chlorotoxin (BmK CT) inhibits the invasion and metastasis of glioma cells and the possibility of I-131-labeled BmK CT (I-131-BmK CT) as a novel targeted agent for the treatment of glioma. The impact of BmK CT with and without I-131 radiolabeling on the invasion and metastasis of glioma cells in vitro was studied. Cell viability was assessed using Cell Counting Kit-8 and plate colony formation assays in order to confirm the cytotoxicity of BmK CT and I-131-BmK CT at different concentrations. Transwell invasion and wound-healing assays were conducted in order to investigate the inhibitory effects BmK CT and I-131-BmK CT on cell migration and invasion. Furthermore, western blotting, ELISA immunofluorescence and a gelatin zymography assay were performed to evaluate changes in the protein expression levels of glioma cells following treatment with BmK CT or I-131-BmK CT. The results indicated that BmK CT inhibits the invasion and metastasis of glioma cells via regulation of tissue inhibitor of metalloproteinase-2 expression and that I-131-BmK CT has the potential to be a novel targeted therapeutic drug for glioma.