A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330

作者：Vuillemin Marlene; Grimaud Florent; Claverie Marion; Rolland Sabate Agnses; Garnier Catherine; Lucas Patrick; Monsan Pierre; Dols Lafargue Marguerite; Remaud Simeon Magali^*; Moulis Claire^*

来源：Carbohydrate Polymers, 2018, 179: 10-18.

DOI：10.1016/j.carbpol.2017.09.056

• Dextran
• Bacterial polysaccharide
• Dextransucrase
• Glucansucrase
• Oenococcus kitaharae

A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 s(-1) and a half-life time of 111 h at 30 degrees C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 x 10(9) g.mol(-1) that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.

• 出版日期2018-1-1