In Vivo and In Vitro Testing With rAni s 1 Can Facilitate Diagnosis of Anisakis simplex Allergy

作者：Martinez Aranguren R M; Gamboa P M; Garcia Lirio E; Asturias J; Goikoetxea M J; Sanz M L^*

来源：Journal of Investigational Allergology and Clinical Immunology, 2014, 24(6): 431-438.

• Anisakis simplex allergy
• Acute urticaria
• Ani s 1
• Component-resolved diagnosis
• In vitro test
• BAT
• ISAC
• ELISA

Background: Traditional diagnostic tests such as skin prick tests (SPT) and specific IgE (sIgE) against whole Anisakis simplex extract have low specificity. Consequently, allergy to A simplex is overdiagnosed. %26lt;br%26gt;Objective: Our aim was to compare tests used in component-resolved diagnosis. %26lt;br%26gt;Methods: We evaluated 34 patients with allergy to A simplex, 15 patients with acute urticaria who were sensitized to A simplex but had no clinical history of allergy to A simplex, and 10 patients allergic to seafood. SPT, sigE (ELISA and ISAC-112), and the basophil activation test (BAT) were performed with A simplex whole extract and the molecular components rAni s 1, rAni s 3, and nPen m 1. Sensitivity and specificity were calculated and compared with different cutoffs. %26lt;br%26gt;Results: With the A simplex whole extract, SPT, sIgE, and BAT yielded specificity values of 72%, 68%, and 70%, respectively, with a cutoff (wheal size) of 11.2 mm, an sIgE value of 7.9 kU(A)/L, and a stimulation index of 1.9. Specificity increased to 100% using the molecular component rAni s 1 with SPT, sIgE by ELISA, and ISAC-112. Neither rAni s 3 sensitization nor cross-reactivity with Pen m 1 was observed in patients sensitized to A simplex. %26lt;br%26gt;Conclusion: rAni s 1 is recognized by 100% of our patients and is able to distinguish between patients allergic to A simplex and patients with acute urticaria who are sensitized to A simplex but have no clinical history of allergy to this parasite.

• 出版日期2014