科研之友
微信
新浪微博
Facebook
分享链接
摘要
Negatively charged bacteria combined with positively charged alkaline dye rhodamine 6G (Rh6G) in NaH2PO4-Na2HPO4 buffer solution pH 7.4, by electrostatic interaction. The dyed bacteria exhibited a strong fluorescence peak at 552 nm and fluorescence intensity was directly linear to Escherichia coli (E. coli), Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aureus) concentrations in the range of 7.06 x 10(4) to 3.53 x 10(7), 4.95 x 10(5) to 2.475 x 10(8) and 32.5 to 16250 colony forming unit/mL (cfu/mL) respectively, with detection limits of 3.2 x 10(4) cfu/mL E. coli, 2.3 x 10(5) cfu/mL B. subtilis and 16 cfu/mL S. aureus, respectively. Samples were cultured for 12 h, after which the linear detection range for E. coli was 2 to 88 cfu/mL. This simple, rapid and sensitive method was used for the analysis of water and drinking samples.
- 出版日期2016-6
- 单位广西师范大学
