Decreased contractile response of peripheral arterioles to serotonin after CPB in patients with diabetes

作者：Sabe Sharif A; Feng Jun; Liu Yuhong; Scrimgeour Laura A; Ehsan Afshin; Sellke Frank W^*

来源：Surgery, 2018, 164(2): 288-293.

DOI：10.1016/j.surg.2018.03.007

Background: Regulation of coronary vasomotor tone by serotonin is significantly changed after cardio-plegic arrest and reperfusion. The current study investigates whether cardiopulmonary bypass may also affect peripheral arteriolar response to serotonin in patients with or without diabetes.
Methods: Human peripheral microvessels (90-180 mu m diameter) were dissected from harvested skeletal muscle tissues from diabetic and non-diabetic patients before and after cardiopulmonary bypass and cardiac surgery (n = 8/group). In vitro contractile response to serotonin was assessed by videomicroscopy in the presence or absence of serotonin alone (10(-9)-10(-5)M) or combined with the selective serotonin 1B receptor (5-HT1B) antagonist, SB224289 (10(-6)M). 5-HT1A/1B protein expression in the skeletal muscle was measured by Western-blot and immunohistochemistry.
Results: There were no significant differences in contractile response of peripheral arterioles to serotonin (10(-5)M) pre-cardiopulmonary bypass between diabetic and non-diabetic patients. After cardiopulmonary bypass, contractile response to serotonin was significantly impaired in both diabetic and non-diabetic patients compared to their pre-cardiopulmonary bypass counterparts (P < .05). This effect was more pronounced in diabetic patients than non-diabetic patients (P < .05 versus non-diabetic). The contractile response to serotonin was significantly inhibited by the 5-HT1B antagonist in both diabetic and nondiabetic vessels (P < .05 versus serotonin alone). There were no significant differences in the expression/distribution of 5-HT1A/1B between non-diabetic and diabetic groups or between pre- versus post-cardiopulmonary bypass vessels.
Conclusions: Cardiopulmonary bypass is associated with decreased contractile response of peripheral arterioles to serotonin and this effect was exaggerated in the presence of diabetes. Serotonin-induced contractile response of the peripheral arterioles was via 5-HTIB in both diabetic and non-diabetic patients.

• 出版日期2018-8