Microbacterium mangrovi sp nov., an amylolytic actinobacterium isolated from mangrove forest soil

作者：Lee Learn Han^*; Azman Adzzie Shazleen; Zainal Nurullhudda; Eng Shu Kee; Ab Mutalib Nurul Syakima; Yin Wai Fong; Chan Kok Gan

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 10): 3513-3519.

DOI：10.1099/ijs.0.062414-0

Strain MUSC 115(T) was isolated from mangrove soil of the Tanjung Lumpur river in the state of Pahang, Peninsular Malaysia. Cells of this strain stained Gram-positive and were non-spore-forming, short rods that formed yellowish-white colonies on different agar media. The taxonomy of strain MUSC 115(T) was studied by a polyphasic approach, and the organism showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Microbacterium. The cell-wall peptidoglycan was of type B2 beta, containing the amino acids ornithine, alanine, glycine, glutamic acid and homoserine. The muramic acid was of the N-glycolyl form. The predominant menaquinones detected were MK-12, MK-13 and MK-11. The polar lipids consisted of phosphatidylglycerol, phosphoglycolipid, diphosphatidylglycerol, two unidentified lipids, three unidentified phospholipids and four unidentified glycolipids. The major fatty acids of the cell membrane were anteiso-C-15:0 and anteiso-C-17:0. The whole-cell sugars detected were ribose, glucose, mannose and galactose. Based on the 16S rRNA gene sequence, strain MUSC 115(T) showed the highest sequence similarity to Microbacterium immunditiarum SK 18(T) (98.1%), M. ulmi XIL02(T) (97.8 %) and M. arborescens DSM 20754(T) (97.5 %) and lower sequence similarity to strains of other species of the genus Microbacterium. DNA DNA hybridization experiments revealed a low level of DNA DNA relatedness (less than 24%) between strain MUSC 115(T) and the type strains of closely related species. Furthermore, BOX-PCR fingerprint comparison also indicated that strain MUSC 115(T) represented a unique DNA profile. The DNA G+C content determined was 70.9 +/- 0.7 mol%, which is lower than that of M. immunditiarum SK 18T. Based on the combination of genotypic and phenotypic data, it is proposed that strain MUSC 115(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium mangrovi sp. nov. is proposed. The type strain is MUSC 115(T) (=MCCC 1K00251(T)=DSM 28240(T)= NBRC 110089(T)).

• 出版日期2014-10