Antigen detection with thermosensitive hydrophilicity of poly(N-isopropylacrylamide)-grafted poly(vinyl chloride) fibrous mats

作者：Guo, Jian-Wei; Lin, Zhen-Yu; Huang, Bohr-Ran; Lu, Chien-Hsing^*; Chen, Jem-Kun^*

来源：Journal of Materials Chemistry B, 2018, 6(21): 3486-3496.

DOI：10.1039/c8tb00870a

• SURFACE-INITIATED POLYMERIZATION
• IMPROVE BLOOD COMPATIBILITY
• BREAST-CANCER RECURRENCE
• CLICK CHEMISTRY
• POLY(ETHYLENE GLYCOL)
• CHEMICAL-MODIFICATION
• CARBON NANOTUBES
• ULTRATHIN FILMS
• ACRYLIC-ACID
• NITRIC-OXIDE

Poly(vinyl chloride) (PVC) was electrospun as fibrous mats to treat with NaN3. The secondary chlorines of PVC were modified to generate azido-terminated electrospun PVC fibrous mats (EPFMs). Sequentially, propargyl-terminated poly(N-isopropylacrylamide) (PNIPAAm) was further synthesized and grafted onto the azido-terminated EPFMs via a click reaction resulting in a scale-like structure on the fibers. The static water contact angles (SWCAs) of the grafted EPFMs reached 0 and 140 degrees when the working temperatures were 25 and 45 degrees C, respectively. In contrast to the grafts on a smooth surface, the change in SWCA from 25 to 45 degrees C was enhanced significantly. EPFMs with PNIPAAm grafts could immobilize an antibody (antiHA), and they could be used for an immunosorbent assay. After coupling with an antigen (HRP-HA) at 80 ng mL(-1) for 8 min, the hydrophobicity of EPFMs with PNIPAAm grafts disappeared completely at 45 degrees C. In addition, the grafted EPFMs exhibited a much more dark blue color than those without the PNIPAAm grafts. SWCA below 10 degrees at 45 degrees C could be exploited as an index to determine the limit of detection (LOD) as 80 ng mL(-1). The immunosorbent assay of EPFMs with PNIPAAm grafts experimentally exhibited high potential in a simple set-up for biosensing due to the unique sensitivity and selectivity of the grafted EPFMs.

• 出版日期2018-6-7
• 单位广东工业大学