Anti-complementary activity of flavonoids from Gnaphalium affine D. Don

作者：Xi, Zhongxin; Chen, Wansheng; Wu, Zhijun; Wang, Yan; Zeng, Peiyuan; Zhao, Guijun; Li, Xia; Sun, Lianna^*

来源：Food Chemistry, 2012, 130(1): 165-170.

DOI：10.1016/j.foodchem.2011.07.025

• Gnaphalium affine D. Don
• Anti-complement
• Acylated flavonol glycosides
• Apigenin 4 '-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside
• Luteolin 4 '-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside
• Quercetin 4 '-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside

Gnaphalium affine D. Don, is used as a vegetable as well as a folk medicine in China for its anti-inflammatory, antitussive, and expectorant activities. Phytochemical investigation of the ethyl acetate (EtOAc) fraction of this plant led to isolation of three new acylated flavonol glycosides, apigenin 4'-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside, luteolin 4'-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside, and quercetin 4'-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside, together with 24 known compounds. The structures of these compounds were determined by spectroscopic methods. All compounds were evaluated for their anti-complementary activity on the classical pathway of the complement system in vitro, and luteolin 4'-O-beta-D-(6 ''-E-caffeoyl)-glucopyranoside exhibited highest anti-complementary activity with an IC50 value of 0.045 +/- 0.005 mg/ml. This is the first report of anti-complementary activity of G. affine D. Don, which displays the initial evidence that this plant is a potent complement inhibitor.

• 出版日期2012-1-1
• 单位中国人民解放军第二军医大学; 天津市中医药研究院附属医院; 福建中医药大学