Differentiation and Genetic Structure of Sclerophoma pythiophila Strains on Pinus sylvestris in Poland

作者：Kraj Wojciech^*

来源：Journal of Phytopathology, 2009, 157(7-8): 400-406.

DOI：10.1111/j.1439-0434.2008.01497.x

• Sclerophoma pythiophila
• Pinus sylvestris
• endophytes
• genetic variation
• random amplified microsatellites

Between 1996 and 2006, 97 strains of Sclerophoma pythiophila were isolated from 3 to 10-year-old trees of Pinus sylvestris from three regions of Poland differing by climatic conditions and geographic location. On the basis of 56 random amplified microsatellites (RAMS) markers obtained with the use of five primers, very high level of genetic variability of strains (mean Jaccard's coefficient 0.56) was ascertained. Among the analysed markers only one was completely monomorphic, whereas six were monomorphic in 90%. Greater genetic similarity was ascertained for strains in southern Poland (0.58) in comparison with strains deriving from northern Poland (0.52). Correlation between the percentage share of polymorphic loci and the average annual temperature of places of strain isolation (r = 0.62) were shown, as well as the number of days with snow cover (r = 0.79). On the basis of the amova analysis, it was ascertained that 11.6% of genetic variability was located between regions of the strains origin and 88.4% within the regions.

• 出版日期2009-8