ASSOCIATION OF XRCC3 GENE POLYMORPHISMS WITH EARLY NORMAL TISSUE ADVERSE EVENTS AFTER PELVIC IRRADIATION FOR GYNECOLOGICAL MALIGNANCIES

作者：Encheva Elitsa^*; Yordanova Iglika; Lakov Teodor; Savov Aleksei; Kremensky Ivo; Mitev Vanio; Kaneva Radka; Hadjieva Tatjana

来源：Comptes Rendus de l Academie Bulgare des Sciences, 2012, 65(3): 403-410.

• Pelvic irradiation
• radiation adverse events
• DNA repair genes SNPs
• XRCC3
• predictive method

The aim of the study was to investigate the possible association of DNA repair gene polymorphisms and early radiation normal tissue adverse events in gynecological cancer patients. 109 patients with cervical and endometrial cancer received a conventional external pelvic irradiation after a radical surgery to a 50 Gy total dose. A four fields %26quot;box%26quot; technique was applied with 2D planning. The acute normal tissue adverse events were graded according to CTCAE v 3.0. From all normal tissue adverse event scores the highest score named %26quot;summarized clinical radiosensitivity%26quot; was selected for a statistical analysis. DNA was isolated from blood samples obtained prior to the beginning of radiotherapy. The genotyping of XRCC3 (241 Thr/Met, IVS5-14 17.893) SNPs was clone by PCR-RFLP. %26lt;br%26gt;109 patients completed the treatment course with no interruption. The majority of the patients developed grade 1 and 2 adverse events. 2% of the patients showed no early radiation adverse events when grades 4-5 were not recorded. Statistically significant correlation between the XRCC3 Codon 241 Thr/Met genotype and the degree of skin reactions was found in the analysis of patients divided into two (0-1 grade and 2-3 grade) and three groups (0 and 2-3 grade) (Fisher exact test, p %26lt; 0.05). In genotype T/T carriers 0-1 grade skin reactions prevail. XRCC3 Codon IVS5-14 (A %26gt; G) was not found to be associated with early radiation adverse events. The conclusion of the present study is that polymorphic T allele of XRCC3 Codon 241 Thr/Met (C %26gt; T) diminishes the risk of development of early moderate and severe adverse skin radiation events.

• 出版日期2012