Fast Diagnostics of BRAF Mutations in Biopsies from Malignant Melanoma

作者：Huber Francois; Lang Hans Peter; Gatz Katharina; Rimoldi Donata; Meyer Ernst; Gerber Christoph

来源：Nano Letters, 2016, 16(9): 5373-5377.

DOI：10.1021/acs.nanolett.6b01513

• Nanomechanical microcantilever
• biosensor
• malignant melanoma
• skin cancer

According to the American skin cancer foundation, there are mote new eases of skin cancer than the combined incidence of cancers of the breast prostate, lung, and colon, each year, and malignant melanoma represents its deadliest form. About 50% of all cases are characterized by a particular mutation BRAF(V600E) in the BRAF (Rapid Acceleration of Fibrosarcoma gene B) gene. Recently developed highly specific drugs are able to fight BRAF(V600E) imitated tumors but,require diagnostic tools for fast and reliable mutation detection to warrant treatment efficiency. We completed a preliminary clinical trial applying cantilever array sensors to demonstrate identification of a BRAF(V600E) single point mutation using total RNA obtained from biopsies of metastatic melanoma of diverse sources (surgical material either frozen or fixated with formalin and embedded in paraffin). The method is faster than the standard Sanger or pyrosequencing methods and. comparably sensitive as next-generation sequencing. Processing time from biopsy to diagnosis is below 1 day and does not require PCR amplification, sequencing, and labels.

• 出版日期2016-9